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About This Item
NACRES:
NA.77
UNSPSC Code:
12352200
Product Name
PDE9A2 Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
specific activity
≥97 pmol/min-μg
mol wt
87.7 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE9A(5152)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human PDE9A2 (GenBank Accession No. NM_001001567), full length with N-terminal GST tag, MW = 87.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Other Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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