SRP0277
PDE10A1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
别名:
HSPDE10A, Phosphodiesterase 10A1, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
≥2851 pmol/min-μg
Assay:
≥60% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥60% (SDS-PAGE)
form
aqueous solution
specific activity
≥2851 pmol/min-μg
mol wt
116 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE10A(10846)
General description
Human PDE10A1 (GenBank Accession No. NM_006661), full length with N-terminal GST tag, MW = 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable