recombinant
expressed in baculovirus
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
46.9 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SPHK1(8877)
General description
Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min