SRP0320
JMJD2b active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)
Synonym(s):
KDM4B, lysine (K)-specific demethylase 4B
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
83 kDa
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
83 kDa
packaging
pkg of 100 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... KDM4B(23030)
General description
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.
Physical form
Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.
Preparation Note
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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