SRP0373
CDK4/Cyclin D1 active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)
Synonym(s):
BCL1, CCND1, Cell division protein kinase 4, Cyclin-dependent kinase 4, PRAD1, PSK-J3
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
29 kDa (Cyclin D1)
34 kDa (CDK4)
packaging
pkg of 20 μg
solubility
water: soluble
NCBI accession no.
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCND1(595), CDK4(1019)
General description
Research area: Apoptosis
Cyclin D1 (CCND1) is a proto-oncogene. It is one of the three D-type cyclins. The gene encoding it is localized on human chromosome 11q13. Cyclin dependent kinase-like 4 (CDKL4) is part of the cyclin-dependent protein kinase (CDK) family. The gene encoding it is localized on human chromosome 12q14-15.
Cyclin D1 (CCND1) is a proto-oncogene. It is one of the three D-type cyclins. The gene encoding it is localized on human chromosome 11q13. Cyclin dependent kinase-like 4 (CDKL4) is part of the cyclin-dependent protein kinase (CDK) family. The gene encoding it is localized on human chromosome 12q14-15.
Recombinant, full length human CDK4 (Genbank Accession No. NM_000075), amino acids 16-271, with C-terminal His-tag and modifications (amino acids 43G-47G were replaced with EE, and T172A), MW=34 kDa, and human Cyclin D1 (Genbank accession number NM_053056), MW 29 kDa, were co-expressed in a Baculovirus-infected Sf9 cells expression system.
Biochem/physiol Actions
Cyclin D1 (CCND1) regulates the Wnt signaling pathway. It also regulates the progression of the cell cycle from the G1 to S phase in many cell types. CCND1 phosphorylates and inactivates retinoblastoma (Rb) protein. Cyclin D1 has been associated with mantle cell lymphoma. This protein is overexpressed in multiple cancers like hepatocellular carcinoma. Cyclin dependent kinase-like 4 (CDKL4) has been associated with various tumors and colorectal cancer. The protein regulates apoptosis of nucleated cells by modifying transcriptional regulation of genes governing cell proliferation and cell death. Like CCND1, it also modulates the progression of cycle from G1 to S phase.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Bioinformatics prediction and experimental validation of microRNA-20a targeting Cyclin D1 in hepatocellular carcinoma
Hamzeh Karimkahnloo
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(4) (2017)
High-throughput RNAi screening of human kinases identifies predictors of clinical outcome in colorectal cancer patients treated with oxaliplatin
Moubin Lin
Oncotarget, 6(18), 16774-16785 (2015)
Impact of cyclin-dependent kinase CDK4 inhibition on eryptosis.
Lang E
Cellular Physiology and Biochemistry, 37(3), 1178-1186 (2015)
Association Between Polymorphism rs678653 in Human Cyclin D1 Gene (CCND1) and Susceptibility to Cancer: A Meta-Analysis
Xichao Dai
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 863-874 (2016)
miR-16 induction after CDK4 knockdown is mediated by c-Myc suppression and inhibits cell growth as well as sensitizes nasopharyngeal carcinoma cells to chemotherapy.
Jiang Q
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 37(2), 2425-2433 (2016)
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