Skip to Content
Merck
CN

SRP0416

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

Synonym(s):

T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.32
UNSPSC Code:
12352204

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

T4 Beta-glucosyltransferase, recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

biological source

Escherichia coli

recombinant

expressed in E. coli

assay

≥83% (SDS-PAGE)

form

aqueous solution

mol wt

41.6 kDa

packaging

pkg of 100 μg

NCBI accession no.

NP_049658

UniProt accession no.

P04547

shipped in

dry ice

storage temp.

−70°C

Gene Information

bacteriophage T4 ... B-GT(1258765)

Application

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Biochem/physiol Actions

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.

General description

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

Physical form

Formulated in 200 mM imidazole and 20% glycerol.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
7200010111
72000-210111

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli.
S R KORNBERG et al.
The Journal of biological chemistry, 236, 1487-1493 (1961-05-01)
Bacteriophage T4 genome.
Miller ES
Microbiology and Molecular Biology Reviews, 67(1), 86-156 (2003)
T4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism.
Morera S
Journal of Molecular Biology, 292(3), 717-730 (1999)
Luis A Ortega-Ramirez et al.
Antibiotics (Basel, Switzerland), 9(3) (2020-03-04)
The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity
Chun-Xiao Song et al.
Nature biotechnology, 29(1), 68-72 (2010-12-15)
In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. Here we present a method for determining the genome-wide distribution
View All Related Papers

Articles

Glycosyltransferases: Tools for Synthesis and Modification of Glycans

Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service