SRP0416
T4 Beta-glucosyltransferase
recombinant, expressed in E. coli, ≥83% (SDS-PAGE)
Synonym(s):
T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352204
Assay:
≥83% (SDS-PAGE)
Biological source:
Escherichia coli
Recombinant:
expressed in E. coli
biological source
Escherichia coli
recombinant
expressed in E. coli
assay
≥83% (SDS-PAGE)
form
aqueous solution
mol wt
41.6 kDa
packaging
pkg of 100 μg
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
bacteriophage T4 ... B-GT(1258765)
General description
T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.
Application
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Biochem/physiol Actions
T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.
Physical form
Formulated in 200 mM imidazole and 20% glycerol.
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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