SRP0417
BirA
recombinant, expressed in E. coli, ≥65% (SDS-PAGE)
Synonym(s):
BirA Biotin Ligase
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352204
recombinant
expressed in E. coli
assay
≥65% (SDS-PAGE)
form
aqueous solution
mol wt
37 kDa
packaging
pkg of 100 μg
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
General description
Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones.
Physical form
Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3.
Kobza K, et al.
Bmb Reports, 41, 310-315 (2008)
Vandana Chakravartty et al.
Journal of bacteriology, 194(5), 1113-1126 (2012-01-03)
Transcription of the Escherichia coli biotin (bio) operon is directly regulated by the biotin protein ligase BirA, the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein
Functional versatility of a single protein surface in two protein:protein interactions.
Adikaram PR and Beckett D
Journal of Molecular Biology, 419, 223-233 (2012)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SRP0417-100UG | 04061837197703 |