SRP0707
PAD Cocktail, Active
recombinant
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~95-100 kDa
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
mol wt
~95-100 kDa
concentration
0.5 μg/μL (0.1 ug/uL each PAD)
accession no.
BC025718, BC049947, NM_013358, NM_016233, NM_207421
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PADI1(29943), PADI2(11240), PADI3(51702), PADI4(23569), PADI6(353238)
Application
Useful for citrullination and Western blot.
Biochem/physiol Actions
The five full length recombinant PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.
Preparation Note
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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