SRP0725
MLK4, Active
PRECISIO® Kinase, recombinant
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~61 kDa
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
15-25 nmol/min·mg (radiometric)
mol wt
~61 kDa
concentration
0.1 μg/μL
accession no.
NM_032435
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MLK4(84451)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human MLK4 (114-420) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas (1). MLK4 is a negative regulator of TLR4 (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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