重组
expressed in baculovirus infected Sf9 cells
方案
70-95% (SDS-PAGE)
表单
frozen liquid
比活
15-25 nmol/min·mg (radiometric)
分子量
~61 kDa
浓度
0.1 μg/μL
登记号
NM_032435
运输
dry ice
储存温度
−70°C
基因信息
human ... MLK4(84451)
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant human MLK4 (114-420) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas (1). MLK4 is a negative regulator of TLR4 (2).
制备说明
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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