recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
4.0-6.0 nmol/min·mg (radiometric)
mol wt
~74 kDa
concentration
0.1 μg/μL
accession no.
NM_007174
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... STK21(11113)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human STK21 (CIT) (1-449) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK21 is a serine/threonine-protein kinase that functions in cell division and also to promote efficient cytokinesis. STK21 is a novel molecular mechanism for a subset of human malformation syndromes of the central nervous system (1). STK21 is also associated with bipolar disorder and risk for schizophrenia. STK21 is a novel partner for the GTP-bound forms of rho and rac (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable