SRP2009
TFIIF (RAP30+Rap74) human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
BTF4
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About This Item
NACRES:
NA.26
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥80% (SDS-PAGE)
Form:
frozen liquid
Mol wt:
~84 kDa
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
frozen liquid
mol wt
~84 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
400 μg/mL
color
clear colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... GTF2F1(2962), GTF2F2(2963)
Biochem/physiol Actions
The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP34) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The Rap74 subunit of TFIIF can be phosphorylated by TAF250 both in vivo and in vitro. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E. coli s factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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