SRP2016
GAL4 [(1-147) - AH] from Saccharomyces cerevisiae
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
Gal81
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About This Item
NACRES:
NA.26
UNSPSC Code:
12352200
Assay:
≥80% (SDS-PAGE)
Biological source:
Saccharomyces cerevisiae
Recombinant:
expressed in E. coli
Concentration:
300 μg/mL
biological source
Saccharomyces cerevisiae
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
frozen liquid
mol wt
~18.6 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
300 μg/mL
technique(s)
electrophoretic mobility shift assay: suitable
color
colorless to clear
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
Saccharomyces cerevisiae ... GAL4(855828)
Biochem/physiol Actions
Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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M Ptashne et al.
Nature, 346(6282), 329-331 (1990-07-26)
Proteins that activate genes are quite disparate in character; in particular, some work 'universally' and others do not. A simple model can accommodate most of the recently published results.
J Ma et al.
Cell, 48(5), 847-853 (1987-03-13)
We describe the activities of a wide array of deletion mutants of GAL4, a yeast transcriptional activator. We identify two short regions of GAL4, each of which activates transcription when fused to the DNA-binding region of the molecule. Very large
Commitment and activation at pol II promoters: a tail of protein-protein interactions.
B Lewin
Cell, 61(7), 1161-1164 (1990-06-29)
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