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SRP2024

Sigma-Aldrich

PC4, serine mutations human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

MGC102747, P15, PC4, p14

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~14.4 kDa

packaging

pkg of 10 μg

concentration

1000 μg/mL

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SUB1(10923)

Biochem/physiol Actions

Human PC4 is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vitro and in vivo. PC4 strongly binds single stranded DNA and the region essential for the single stranded DNA binding activity was mapped around residue 77. A single amino acid change at position 77 (F to P) abolishes both ds- and ss-DNA binding activity.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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