SRP3016
BMP-4 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
BMP-2B, DVR4
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
5-10 ng/mL ED50
mol wt
23.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BMP4(652)
General description
Recombinant human Bone Morphogenetic Protein‑4 (BMP‑4) is a homodimeric signaling protein expressed in E. coli and supplied as a lyophilized powder for mammalian cell culture research in vitro.
Application
Recombinant BMP‑4 has been used in mammalian cell culture research applications involving stem cell differentiation and lineage‑specific in vitro model systems.
Biochem/physiol Actions
Bone Morphogenetic Protein‑4 (BMP‑4) is a TGF‑β family growth factor that mediates cellular responses through SMAD‑dependent signaling. It is involved in diverse physiological processes including development and maintenance of bone and cartilage during both pre- and postnatal life.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20° C to -80° C.
Other Notes
KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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