SRP3084
IL-1Ra human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
ICIL-1RA, IL-1RN, IRAP
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥98% (HPLC)
≥98% (SDS-PAGE)
≥98% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
17.2 kDa
Product Name
IL-1Ra human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
20-40 ng/mL
mol wt
17.2 kDa
packaging
pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
suitability
suitable for molecular biology
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL1R1(3554)
Application
IL-1Ra human has been used as an IL1 antagonist to block the signaling from the IL1 receptor and verify that IL1 signaling is responsible for invasiveness in CD133-expressing cells. It has also been used as a control in sandwich enzyme-linked immunosorbent assay (ELISA) to measure protein levels.
Biochem/physiol Actions
Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α (interleukin 1 α) and IL-1β (interleukin 1 β) by competitively blocking binding to their type I and type II receptors. Therapeutically, IL-1RA may help in the treatment of sepsis, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Low production of IL-1RA is observed in HIV-1 (human immunodeficiency virus 1)-infected dendritic cells.
The interleukin-1 receptor antagonist (IL-1Ra) functions as a specific and selective competitive inhibitor of IL-1R1, binding to the receptor with high affinity.
General description
Research Area: IMMUNO AND CKS
The interleukin-1 receptor antagonist (IL-1Ra) is part of the IL-1 family and binds to IL-1 receptors without triggering any intracellular response. IL-1Ra, produced by hepatic cells, exhibits characteristics of an acute phase protein. Endogenous IL-1Ra is generated in various experimental animal models of disease, as well as in human autoimmune and chronic inflammatory conditions.
The interleukin-1 receptor antagonist (IL-1Ra) is part of the IL-1 family and binds to IL-1 receptors without triggering any intracellular response. IL-1Ra, produced by hepatic cells, exhibits characteristics of an acute phase protein. Endogenous IL-1Ra is generated in various experimental animal models of disease, as well as in human autoimmune and chronic inflammatory conditions.
The gene IL-1RA (interleukin-1 receptor antagonist) is mapped to human chromosome 2q. It belongs to the IL-1 (interleukin 1) family of proteins. The encoded protein has a TIR (Toll/IL-1 receptor) domain. IL-1RA is produced by corneal epithelial cells, monocytes, neutrophils, macrophages and fibroblasts. It is a naturally occurring inflammatory inhibitor protein. Recombinant human IL-1RA is a 17.2kDa protein consisting of 153 amino acid residues.
Other Notes
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Physical form
Lyophilized from 5mM Tris, pH 8.0 + 25 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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A synthetic mammalian therapeutic gene circuit for sensing and suppressing inflammation
Smole A, et al.
Molecular Therapy, 102-119 (2017)
The molecular basis of nonoxynol-9-induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission.
The Journal of Infectious Diseases, 184, 418-428 (2001)
Association between interleukin-1 receptor antagonist (IL-1ra) gene polymorphism and early and late-onset psoriasis.
Tarlow JK
British Journal of Dermatology, 136, 147-148 (1997)
The effects of interleukin-10 on interleukin-1 receptor antagonist and interleukin-1 beta production in human monocytes and neutrophils.
Jenkins JK
Lymphokine and Cytokine Research, 13, 47-54 (1994)
Recombinant interleukin-1 receptor antagonist (IL-1ra): effective therapy against gram-negative sepsis in rats.
Alexander HR
Surgery, 112, 188-193 (1992)
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