SRP3110
M-CSF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CSF-1, MGI-IM, Macrophage Colony Stimulating Factor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤1.0 ng/mL ED50
mol wt
36.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CSF1(1435)
General description
M-CSF (macrophage colony-stimulating factor) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The protein works through its receptor CSF1R (colony stimulating factor 1 receptor). The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant human M-CSF is a 36.8kDa homodimeric protein containing two 159 amino acid polypeptide subunits.
Application
M-CSF human has been used for the induction of osteoclastogenesis in human peripheral blood mononuclear cells.
Biochem/physiol Actions
M-CSF (macrophage colony-stimulating factor) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. M-CSF also works as a pleiotropic growth factor and plays a crucial role in reproduction and organogenesis. Absence of it can result in reduced growth, neurological and reproductive problems, developmental defects in the mammary gland, bone and pancreas. M-CSF is also involved in the pathogenesis of cancer.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 8.0 + 75 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Other Notes
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The inhibitory effect of microRNA-146a expression on bone destruction in collagen-induced arthritis.
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Bedkowska GE, et al.
Journal of Ovarian Research, 8, 27-27 (2015)
The macrophage colony-stimulating factor, CSF-1.
E R Stanley
Methods in enzymology, 116, 564-587 (1985-01-01)
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Articles
Read article on hematopoietic cytokines and hematopoiesis
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