SRP3154
sFRP-1 human
recombinant, expressed in HeLa cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Synonym(s):
SARP-2, Secreted Apoptosis Related Protein-2, Soluble Frizzled Related Protein-1
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥97% (HPLC), ≥97% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HeLa cells
Mol wt:
31.0-36.0 kDa
biological source
human
recombinant
expressed in HeLa cells
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
potency
0.3-0.5 μg/mL ED50
mol wt
31.0-36.0 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... SFRP1(6422)
General description
The gene SFRP1 (secreted frizzled related protein 1) is mapped to human chromosome 8p22–p12, a region frequently deleted in breast cancer. It is highly expressed in normal breast tissue.
Biochem/physiol Actions
Secreted Frizzled-related protein 1 (SFRP1) is a secreted inhibitor of Wnt signaling pathway. DNA damage or oxidative stress causes cellular senescence, which leads to overexpression of sfrp1. This protein induces senescence phenotypes via the inactivation of Wnt signaling and activation of the retinoblastoma (Rb) pathway. Mutations or aberrant methylation in this gene impair the induction of senescence in several cancers, including breast cancer, bladder cancer, and colorectal tumorigenesis.
Physical form
Lyophilized from 10 mM Sodium Phosphate pH 7.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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