SRP3228
PDGF-AA from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32190102
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
8-10 ng/mL ED50
mol wt
28.7 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... PDGFA(18590)
General description
The PDGFs are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Application
PDGF-AA from mouse has been used as a supplement in the media used for differentiation of neurospheres.
Biochem/physiol Actions
The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA is crucial for optimal wound healing.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
Storage Class
11 - Combustible Solids
wgk
WGK 1
Regulatory Information
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Native and oxidized LDL enhances production of PDGF AA and the surface expression of PDGF receptors in cultured human smooth muscle cells.
Stiko-Rahm A
Arteriosclerosis and Thrombosis, 12, 1099-1109 (1992)
Detection of platelet-derived growth factor (PDGF)-AA in actively healing human wounds treated with recombinant PDGF-BB and absence of PDGF in chronic nonhealing wounds.
Pierce GF
The Journal of Clinical Investigation, 96, 1336-1350 (1995)
Isolation of neural stem cells from the postnatal cerebellum.
Lee A
Nature Neuroscience, 8, 723-729 (2005)
Interleukin-1 mitogenic activity for fibroblasts and smooth muscle cells is due to PDGF-AA.
Raines EW
Science, 243, 393-396 (1989)
An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA.
Demaria M
Developmental Cell, 31, 722-733 (2014)
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