SRP3229
PDGF-BB from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
biological source
mouse
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
1.0-2.0 ng/mL ED50
mol wt
24.4 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
off-white to yellow
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... PDGFB(18591)
General description
PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Application
PDGF-BB from mouse has been used as a chemotactic factor on vascular smooth muscle cell.
Biochem/physiol Actions
The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Heterozygous mutation in PDGFB is associated with primary familial brain calcification. It is also associated with angiogenesis for osteogenesis. PDGFB induces migration of endothelial progenitor cells and mesenchymal stem cells.
Physical form
Lyophilized from 10 mM Sodium Citrate, pH 4.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis.
Xie H, et al.
Nature Medicine, 20, 1270-1278 (2014)
Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor.
Fretto LJ, et al.
The Journal of Biological Chemistry, 268, 3625-3631 (1993)
Arterial smooth muscle cells express platelet-derived growth factor (PDGF) A chain mRNA, secrete a PDGF-like mitogen, and bind exogenous PDGF in a phenotype- and growth state-dependent manner.
Sjolund M, et al.
The Journal of Cell Biology, 106, 403-413 (1988)
Roles of PDGF in animal development.
Hoch RV and Soriano P
Development, 130, 4769-4784 (2003)
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, et al.
The American Journal of Pathology, 163, 869-878 (2003)
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