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SRP4037

Sigma-Aldrich

FGF-2 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

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Synonym(s):
FGF-2, Fibroblast Growth Factor-basic, HBGF-2, Prostatropin
MDL number:
MFCD00081382

biological source

human

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~17.2 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

NM_002006

UniProt accession no.

P09038

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF2(2247)

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General description

The gene FGF2 (fibroblast growth factor 2) is mapped to human chromosome 4q28. FGF-2 is a basic heparin binding growth factor. The recombinant human FGF-2 is a 17.2kDa protein containing 154 amino acid residues. It is also referred to as BFGF (basic fibroblast growth factor).

Application

FGF-2 human has been used:
  • as medium supplement for E9.5 neuroepithelial cell culture
  • as a component in the neurobasal growth medium
  • in growing embryonic neural stem/progenitor cells (NSPCs) in a 2D culture

FGF2 (fibroblast growth factor 2)-human has been used in endothelial colony assay.

Biochem/physiol Actions

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. The FGF2 mRNA is a target of miRNA miR-503.

Physical form

Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 plus 150 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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Zinc deficiency causes neural tube defects through attenuation of p53 ubiquitylation
Li H, et al.
Development, 145(24), dev169797-dev169797 (2018)
Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors.
Peichev M
Blood, 95, 952-958 (2000)
Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
Rybachuk O, et al.
Bio-protocol, 9(4) (2019)
Role of microRNA?503 in the suppression of osteosarcoma cell proliferation and migration via modulation of fibroblast growth factor 2.
Wu B and Bi W
Molecular Medicine Reports, 12, 7433-7438 (2015)
Yang Hu et al.
Molecular medicine reports, 13(1), 49-58 (2015-11-18)
Recent studies regarding regenerative medicine have focused on bone marrow mesenchymal stem cells (BMSCs), which have the potential to undergo neural differentiation, and may be transfected with specific genes. BMSCs can differentiate into neuron‑like cells in certain neurotropic circumstances in
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