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SRP4596

IFN-alpha 1 human

Name: IFN-alpha 1 human
Brand: SIGMA

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

IFN-α 1, IFNA1, Interferon-α

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

MDL number:

MFCD01773209

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

potency

1x10^8 units/mg

mol wt

~19.3 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

NM_024013

UniProt accession no.

P01562

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNA1(3439)

Description

General description

IFN-α1 (interferon α 1) is a type 1 interferon. Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes. The IFN-α1 gene is mapped to human chromosome 9p21.3.

Application

IFN-alpha 1 human has been used:

to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection
as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography
to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation
as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response

Biochem/physiol Actions

IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity. IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins. IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma. The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.

Physical form

Lyophilized without any additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c