SRP4805
Thrombospondin human
recombinant, expressed in baculovirus infected Sf9 cells, ≥98% (SDS-PAGE)
Synonym(s):
THBS, THBS1, TSP, TSP1, Thrombospondin-1
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
~180.0 kDa
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TSP1(7057)
General description
The gene TSP1 (thrombospondin 1) is mapped to human chromosome 15q14. It is a matricellular glycoprotein and can be secreted by many cells. Recombinant human thrombospondin is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Human thrombospondin is purified by proprietary chromatographic techniques.
Application
In rat C6 astroglioma cells, TSP1 (thrombospondin 1)-human has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression. It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
Biochem/physiol Actions
TSP1 (thrombospondin 1) is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. TSP1 expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP1 stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-γ (interferon γ) and TNF-α (tumor necrosis factor α) inhibits TSP1 production in these cells. In endothelial cells, it controls adhesion, migration as well as proliferation. It also exhibits antiangiogenic properties and regulates immune processes. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. It also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. TSP1 has been used as a potential regulator of tumor growth and metastasis. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis. Variants of this gene might be linked with increased risk of autism.
Physical form
Lyophilized from 20 mM sodium phosphate, pH 6.0, 300
mM NaCl and 0.1% BSA.
mM NaCl and 0.1% BSA.
Preparation Note
Reconstitute with water at the concentration 0.1 mg/ml. Avoid freeze-thaw cycles.
Storage Class
13 - Non Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
涉药品监管产品
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Common and rare variants of the THBS1 gene associated with the risk for autism.
Psychiatric Genetics, 24, 235-240 (2014)
Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1.
Ridnour LA
Proceedings of the National Academy of Sciences of the USA, 102, 13147-13152 (2005)
Thrombospondin-1 suppresses wound healing and granulation tissue formation in the skin of transgenic mice.
Streit M
The Embo Journal, 19, 3272-3282 (2000)
Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-?1 toward Fibroblast-like Synovial Cells.
The Journal of Rheumatology, 42, 943-943 (2015)
Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression.
Cell communication and signaling : CCS, 11, doi: 10-doi: 10 (2013)
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