SRP5117
PCNA, His tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MGC8367
Product Name
PCNA, His tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~37 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PCNA(5111)
Biochem/physiol Actions
Proliferating cell nuclear antigen (PCNA) significantly maintains the longevity of mature neutrophil granulocytes. Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma. PCNA supports cell survival by elevating metabolism flux. PCNA expression is known to offer resistance to chemotherapy. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro. PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H.
General description
PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein. PCNA is a homotrimeric protein known to localize in the nucleus. The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it. PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta.
Physical form
Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Hisham Mazal et al.
eLife, 11 (2022-05-27)
Cryogenic optical localization in three dimensions (COLD) was recently shown to resolve up to four binding sites on a single protein. However, because COLD relies on intensity fluctuations that result from the blinking behavior of fluorophores, it is limited to
Proliferating cell nuclear antigen prevents trinucleotide repeat expansions by promoting repeat deletion and hairpin removal
Beaver JM, et al.
DNA Repair, 48(2), 17-29 (2016)
Cytoplasmic proliferating cell nuclear antigen connects glycolysis and cell survival in acute myeloid leukemia
Ohayon D, et al.
Scientific reports, 6(3), 35561-35561 (2016)
Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA.
Hedglin M and Benkovic SJ
Biochemistry, 56(13), 1824-1835 (2017)
Raymond A Poot et al.
Nature cell biology, 6(12), 1236-1244 (2004-11-16)
Chromatin states have to be faithfully duplicated during DNA replication to maintain cell identity. It is unclear whether or how ATP-dependent chromatin-remodelling factors are involved in this process. Here we provide evidence that the Williams syndrome transcription factor (WSTF) is
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