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Merck
CN

SRP6014

HGF human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

Synonym(s):

F-TCF, HGF, HGFB, Hepatopoietin (HPTA), Scatter Factor (SF)

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About This Item

CAS Number:
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥97% (HPLC), ≥97% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in CHO cells
Mol wt:
80.017 kDa
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biological source

human

recombinant

expressed in CHO cells

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

form

lyophilized

mol wt

80.017 kDa

packaging

pkg of 1 mg, pkg of 10 μg

technique(s)

cell culture | embryo: suitable

impurities

Endotoxin, tested

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... HGF(3082)

General description

Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques.

Biochem/physiol Actions

Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors.

Physical form

The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Preparation Note

Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm-1 H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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