SRP6147
Ubiquitin Activating Enzyme E1 human
≥95% (SDS-PAGE)
Synonym(s):
AMCX1, POC20, UBA1, UBE1, Ubiquitin E1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Assay:
≥95% (SDS-PAGE)
Biological source:
human
biological source
human
assay
≥95% (SDS-PAGE)
form
liquid
potency
>3 nM
mol wt
118
packaging
pkg of 50 μg
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... UBA1(7317)
General description
Application
Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.
Biochem/physiol Actions
Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).
Physical form
In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.
Preparation Note
Centrifuge the vial prior to opening.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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