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SRP6258

Granzyme B human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

CCPI, CGL-1, CSP-B, CSPB, CTLA1, CTSGL1, Fragmentin-2, GRB, GZMB, Granzyme B, Granzyme-2, HLP, SECT

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Key Documents

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Product Name

Granzyme B human, recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 26.4 kDa
observed mol wt 35 kDa (DTT-reduced. Protein migrates due to glycosylation. Ile 21 is the predicted N-terminus.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P10144 

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GZMB(3002)

General description

Granzyme B (GZMB) also known as Cathepsin G-like 1 (CTSGL1), Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease), Fragmentin-2, Granzyme-2, Human lymphocyte protein (HLP), SECT, CGL1, CSPB, CTLA1, GRB, which belongs to the peptidase S1 family. Granzyme subfamily GZMB / CTSGL1 contains 1 peptidase S1 domain. The catalytic activity of Granzyme B is “Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-“, and is inactivated by the serine protease inhibitor diisopropyl fluorophosphates. GZMB is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. GZMB seems to be linked to activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution and cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
5H15L72330
5L12L72330
5B13L72330
3M28L72330

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