SRP6270
MMP-2 human
recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)
Synonym(s):
CLG4, CLG4A, MMP-II, MMP2, MONA, TBE-1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
MMP-2 human, recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥92% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 71.8 kDa
observed mol wt 66-71 kDa (DTT-reduced. Ala 30 is the predicted N-terminus.)
packaging
pkg of 10 and 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MMP-2(4313)
Biochem/physiol Actions
Matrix metalloproteinase-2 (MMP-2) cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. It is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. It degrades type IV, V, VII, and X collagens. The protein degrades extracellular matrix proteins and acts on several non-matrix proteins such as big endothelial 1 (bET) and β-type calcitonin gene-related peptide (CGRP) promoting vasoconstriction. MMP-2 also cleaves KISS (metastasis suppressor) at a Gly-|-Leu bond. The protein appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of glycogen synthase kinase-3 β (GSK3β). It has been shown to cleave GSK3β in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to fibroblast growth factor-2 (FGF2) and vitronectin.
General description
Matrix metalloproteinase-2 (MMP-2) which is also known as 72kDa type IV collagenase, 72kDa gelatinase, gelatinase A and CLG4A, belongs to the peptidase M10A family. It contains three fibronectin type-II domains and four hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. The gene encoding this protein is localized on human chromosome 16q12.2.
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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The association between inflammation-related genes and serum androgen levels in men: the prostate, lung, colorectal, and ovarian study.
Meyer TE
Prostate, 72(1), 65-71 (2012)
Increased expression of 72-kd type IV collagenase (MMP-2) in human aortic atherosclerotic lesions.
Li Z
The American Journal of Pathology, 148(1), 121-128 (1996)
Matrix metalloproteinase-2 and myocardial oxidative stress injury: beyond the matrix.
Kandasamy AD
Cardiovascular Research, 85(3), 413-423 (2010)
Disruption of angiogenesis by PEX, a noncatalytic metalloproteinase fragment with integrin binding activity.
Brooks PC,
Cell, 92(3), 391-400 (1998)
Activated interstitial myofibroblasts express catabolic enzymes and mediate matrix remodeling in myxomatous heart valves.
Rabkin E
Circulation, 104(21), 2525-2532 (2001)
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