SRP6455
EpCAM/CD326 human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK1, TACSTD1, TROP1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 29 kDa (after removal of signal peptide.), observed mol wt 33-36 kDa by SDS-PAGE
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 29 kDa (after removal of signal peptide.), observed mol wt 33-36 kDa by SDS-PAGE
packaging
pkg of 10 and 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... EPCAM(4072)
General description
DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.
Epithelial cell adhesion molecule (EpCAM) is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A (mAb) antigen. The gene encoding EpCAM is localized on human chromosome 2p21.
Biochem/physiol Actions
Epithelial cell adhesion molecule (EpCAM) is intricately linked with the cadherin-catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The protein has several cellular functions, like epithelial-specific cell-adhesion, cell signaling, migration, proliferation and differentiation. It is expressed in most epithelial malignancies (like epithelial ovarian cancer) and was reported as a tumor marker or a candidate of molecular targeting therapy. EpCAM cross signaling with N-cadherin involves phosphoinositide-3-kinase (Pi3K), resulting in the abrogation of the cadherin adhesion complexes in epithelial cells.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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Overexpression of epithelial cell adhesion molecule (Ep-CAM) is an independent prognostic marker for reduced survival of patients with epithelial ovarian cancer.
Spizzo G
Gynecologic Oncology, 103(2), 483-488 (2006)
Cadherins are regulated by Ep-CAM via phosphaditylinositol-3 kinase.
Winter MJ
Molecular and Cellular Biochemistry, 302(1-2), 19-26 (2007)
Epithelial cell adhesion molecule expression (CD326) in cancer: a short review.
Patriarca C
Cancer Treatment Reviews, 38(1), 68-75 (2012)
The evolving roles of canonical WNT signaling in stem cells and tumorigenesis: implications in targeted cancer therapies
Ke Yang
Laboratory Investigation; a Journal of Technical Methods and Pathology, 96, 116-136 (2016)
The Epithelial Cell Adhesion Molecule (Ep-CAM) as a Morphoregulatory Molecule Is a Tool in Surgical Pathology
Manon J. Winter
The American Journal of Pathology, 163(6), 2139-2148 (2003)
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