SRP6472
Transferrin human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
DKFZp781D0156, PRO1557, PRO2086, TF
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized
Mol wt:
calculated mol wt 76 kDa, observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 76 kDa, observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)
packaging
pkg of 100 μg, pkg of 20 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TF(7018)
General description
Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.
Application
To supplement neurosphere culture medium and sphere differentiation medium.
Biochem/physiol Actions
Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
Regulatory Information
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Roles of the basic helix-loop-helix genes Hes1 and Hes5 in expansion of neural stem cells of the developing brain.
Ohtsuka T
The Journal of Biological Chemistry, 276, 30467-30474 (2001)
Iron transport and storage proteins.
P Aisen et al.
Annual review of biochemistry, 49, 357-393 (1980-01-01)
Phylogenetic analyses uncover a novel clade of transferrin in nonmammalian vertebrates.
Mohd-Padil H
Molecular Biology and Evolution, 30, 894-905 (2013)
Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor alpha.
Brandt JM
The Journal of Biological Chemistry, 273, 23786-23792 (1998)
Anion binding by human lactoferrin: results from crystallographic and physicochemical studies.
Shongwe MS
Biochemistry, 31, 4451-4458 (1992)
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