SRP6586
Histone H3 (1-136) human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym(s):
Histone H3/I, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/j, Histone H3/k, Histone H3/l
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
15.5 kDa (1-136 aa)
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
15.5 kDa (1-136 aa)
packaging
pkg of 100 and 250 μg
Gene Information
human ... HIST1H3A(8350)
shipped in
dry ice
storage temp.
−70°C
General description
The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)2. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).
Biochem/physiol Actions
Histone proteins are basic building blocks of chromatin. Changes in the circulating H3 levels are associated with death in sepsis patients. Post-translational modifications of histone tails control chromatin organization and gene transcription. Trimethylated histone H3 lysine 4 (H3K4me3) is present at actively transcribed genes and it helps in promotion of gene expression. Histone H3K36 trimethylation (H3K36me3) is lost in various cancers and is linked with poor patient outcome. Dimethylated H3K9 (H3K9me2) is mainly present in euchromatin and is linked with repressed promoter areas. Histone methyltransferase G9a is responsible for hypoxic stress-mediated H3K9me2 in mammalian cells.
Physical form
Lyophilized powder. Recommended buffer is 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Extracellular histone H3 levels are inversely correlated with antithrombin levels and platelet counts and are associated with mortality in sepsis patients.
Wildhagen KC, et al.
Thrombosis Research, 136, 542-542 (2015)
Inhibiting WEE1 Selectively Kills Histone H3K36me3-Deficient Cancers by dNTP Starvation.
Pfister SX, et al.
Cancer Cell, 28, 557-557 (2015)
Suv39h-mediated histone H3 lysine 9 methylation directs DNA methylation to major satellite repeats at pericentric heterochromatin.
Lehnertz B, et al.
Current Biology, 13, 1192-1192 (2003)
Genome-wide analysis of histone H3 lysine 4 trimethylation in peripheral blood mononuclear cells of minimal change nephrotic syndrome patients.
Zhang L, et al.
American Journal of Nephrology, 30, 505-505 (2009)
Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA.
Arents G and Moudrianakis EN
Proceedings of the National Academy of Sciences of the USA, 90, 10489-10489 (1993)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service