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SRP8012

Sigma-Aldrich

Jagged-1 (human): FC (human)

recombinant, expressed in HEK 293 cells, >90% (SDS-PAGE)

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Synonym(s):
JAG1, Protein jagged-1, Tweak-receptor, hJ1
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

>90% (SDS-PAGE)

form

liquid

mol wt

~150 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

≥0.2 mg/mL

technique(s)

ligand binding assay: suitable

impurities

<0.1 EU/μg endotoxin, tested

UniProt accession no.

P78504

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... JAG1(182)

General description

Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF).
Jagged-1, encoded by the gene JAG1, is a Serrate homolog found in vertebrates. It is expressed by both primitive and mature human hematopoietic cells. The encoded protein contains EGF (epidermal growth factor) repeats, transmembrane domain and a cysteine-rich region in between the two.

Application

Jagged-1 has been used to activate Notch1 signaling pathway that modulates cardiac AMPK (5′ AMP-activated protein kinase) signaling cascade. Jagged-1 (human): FC (human) has been used to determine the cardiac substrate (fatty acid and glucose) metabolism in working heart model.

Biochem/physiol Actions

The encoded protein is a transmembrane-type ligand for Notch family receptor and is capable of inducing Notch signaling, which participates in embryogenesis, tissue regeneration, and carcinogenesis. Jagged-1 is a WNT-dependent Notch signaling activator that is involved in the maintenance of homeostasis of stem and progenitor cells. Jagged proteins are found to be involved in development and carcinogenesis. Overexpression of Jagged-1 has been observed in cervical and breast cancers.

Physical form

Solution in PBS.

Other Notes

Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG1.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival.
Reedijk M
Cancer Research, 65, 8530-8537 (2005)
The notch ligand jagged-1 represents a novel growth factor of human hematopoietic stem cells.
Karanu FN
The Journal of Experimental Medicine, 192, 1365-1372 (2000)
Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation.
Purow BW
Cancer Research, 65, 2353-2363 (2005)
Notch ligand, JAG1, is evolutionarily conserved target of canonical WNT signaling pathway in progenitor cells.
Katoh M and Katoh M
International Journal of Molecular Medicine, 17, 681-685 (2006)
B Varnum-Finney et al.
Blood, 91(11), 4084-4091 (1998-05-30)
We examined the expression of two members of the Notch family, Notch-1 and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (Lin-Sca-1+c-kit+). The Notch ligand, Jagged-1, was not detected
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