SRP8024
TRAIL, Soluble human
recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)
Synonym(s):
Apo-2L, CD253, CD339, TRAIL
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352200
Product Name
TRAIL, Soluble human, recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
tag
His tagged
assay
>98% (SDS-PAGE)
form
liquid
mol wt
~26 kDa
packaging
pkg of 20 μg
concentration
≥0.2 mg/mL
technique(s)
activity assay: suitable
cell culture | mammalian: suitable
transfection: suitable
impurities
<0.01 EU/μg endotoxin, tested
color
clear
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TNFSF10(8743)
Application
TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.
Biochem/physiol Actions
Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).
General description
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.
Other Notes
The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
Physical form
Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Intracranial AAV-sTRAIL combined with lanatoside C prolongs survival in an orthotopic xenograft mouse model of invasive glioblastoma.
Crommentuijn MH
Molecular Oncology, 10(4), 625-634 (2016)
Association of TRAIL and Its Receptors with Large-Artery Atherosclerotic Stroke.
Pan X
PLoS ONE, 10(9), e0136414-e0136414 (2015)
Association between endometriosis and polymorphisms in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), TRAIL receptor and osteoprotegerin genes and their serum levels.
Kim H
Archives of Gynecology and Obstetrics, 286(1), 147-153 (2012)
Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway
Dmitriy Ovcharenko
Cancer Research (2007)
Tumor Necrosis Factor-a Blocks Apoptosis in Melanoma Cells when BRAF Signaling Is Inhibited
Vanessa C Gray-Schopfer
Cancer Research (2007)
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