SRP9001
Leukemia Inhibitory Factor human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, liquid, suitable for cell culture
Synonym(s):
Differentiation-stimulating factor, Emfilermin, LIF, Melanoma-derived LPL inhibitor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Product Name
Leukemia Inhibitory Factor, human, recombinant, expressed in HEK 293 cells
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
Assay
≥95% (SDS-PAGE)
form
liquid
potency
≤0.5 ng/mL M1 cells proliferation inhibition EC50
mol wt
20 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg protein Endotoxin level
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... LIF(3976)
General description
LIF is manufactured using an all-human production system, with no serum.
Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.
Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.
Application
Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.
One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.
Biochem/physiol Actions
Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.
Physical form
This product is supplied as a solution in 0.2 μm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.
Preparation Note
Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Cytokines hold promise for human embryo culture in vitro: results of a randomized clinical trial
Fawzy M, et al.
Fertility and Sterility, 112(5), 849-857 (2019)
Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Xu J et al
Cell Stress & Chaperones, 17(4), 485-493 (2012)
Molecular features of the basal-like breast cancer subtype based on BRCA1 mutation status.
Prat A, et al.
Breast Cancer Research and Treatment, 147(1), 185-191 (2014)
The gp130 receptor cytokine family: regulators of adipocyte development and function.
White UA and Stephens JM
Current Pharmaceutical Design, 17(4), 340-346 (2011)
Pin Li et al.
EMBO molecular medicine, 14(1), e14511-e14511 (2021-11-16)
In the course of our studies aiming to discover vascular bed-specific endothelial cell (EC) mitogens, we identified leukemia inhibitory factor (LIF) as a mitogen for bovine choroidal EC (BCE), although LIF has been mainly characterized as an EC growth inhibitor
Articles
Read article on hematopoietic cytokines and hematopoiesis
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