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STRN50

Sigma-Aldrich

Spectrum™ Plant Total RNA Kit

greener alternative

sufficient for 50 purifications

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Synonym(s):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
NACRES:
NA.52

usage

sufficient for 50 purifications

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

RNA purification: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

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General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum® Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

SabreLite is a registered trademark of Pelican Products, Inc.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Regulatory Information

危险化学品

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Asfaw Degu et al.
BMC plant biology, 19(1), 69-69 (2019-02-13)
Grape leaves provide the biochemical substrates for berry development. Thus, understanding the regulation of grapevine leaf metabolism can aid in discerning processes fundamental to fruit development and berry quality. Here, the temporal alterations in leaf metabolism in Merlot grapevine grown
Neeraj K Dubey et al.
Frontiers in plant science, 8, 1574-1574 (2017-09-29)
RNA silencing refers to diverse mechanisms that control gene expression at transcriptional and post-transcriptional levels which can also be used in parasitic pathogens of plants that Broomrapes (Orobanche/Phelipanche spp.) are holoparasitic plants that subsist on the roots of a variety
Meng Zhang et al.
Journal of experimental botany, 71(3), 951-969 (2019-10-23)
Anther development in flowering plants is highly sensitive to high-temperature (HT) stress. Understanding the potential epigenetic mechanism of anther infertility induced by HT stress in cotton (Gossypium hirsutum L.) is crucial for the effective use of genetic resources to guide
A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.
Singh B
Plant Cell Reports, 28(7), 1023-1032 (2009)
Nikolaj L Hansen et al.
Microbial cell factories, 19(1), 15-15 (2020-01-30)
Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking. To elucidate the celastrol biosynthetic pathway
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Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service