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STRN50

Spectrum™ Plant Total RNA Kit

greener alternative

sufficient for 50 purifications

Synonym(s):

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501

Key Documents

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Product Name

Spectrum™ Plant Total RNA Kit, sufficient for 50 purifications

usage

sufficient for 50 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

test parameters

sample volume: 100 mg

greener alternative category

Aligned,

storage temp.

15-25°C

Quality Level

200

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Application

Spectrum Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
0000393619
0000393619
0000376931
0000376924
0000273906

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A synthetic auxin (NAA) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.
Singh B
Plant Cell Reports, 28(7), 1023-1032 (2009)
Mohammed Al-Issawi et al.
Plant physiology and biochemistry : PPB, 63, 77-81 (2012-12-19)
Wheat is able to cold acclimate in response to low temperatures and thereby increase its frost tolerance and the extent of this acclimation is greater in winter genotypes compared to spring genotypes. Such up-regulation of frost tolerance is controlled by
Jinbu Jia et al.
The Plant cell, 32(12), 3662-3673 (2020-10-21)
In plants, 22-nucleotide small RNAs trigger the production of secondary small interfering RNAs (siRNAs) and enhance silencing. DICER-LIKE2 (DCL2)-dependent 22-nucleotide siRNAs are rare in Arabidopsis (Arabidopsis thaliana) and are thought to function mainly during viral infection; by contrast, these siRNAs
Asfaw Degu et al.
BMC plant biology, 19(1), 69-69 (2019-02-13)
Grape leaves provide the biochemical substrates for berry development. Thus, understanding the regulation of grapevine leaf metabolism can aid in discerning processes fundamental to fruit development and berry quality. Here, the temporal alterations in leaf metabolism in Merlot grapevine grown
Meng Zhang et al.
Journal of experimental botany, 71(3), 951-969 (2019-10-23)
Anther development in flowering plants is highly sensitive to high-temperature (HT) stress. Understanding the potential epigenetic mechanism of anther infertility induced by HT stress in cotton (Gossypium hirsutum L.) is crucial for the effective use of genetic resources to guide
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Articles

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Related Content

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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