T1194
Trizma® Pre-set crystals
BioPerformance Certified, pH 8.5, average Mw 131.4
Synonym(s):
Tris crystals
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
grade
BioPerformance Certified
Quality Level
form
crystalline powder
mol wt
average Mw 131.4
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)
pH
8.22 (37 °C, 0.1 M)(lit.), 8.50 (25 °C, 0.1 M)(lit.), 8.5, 9.09 (5 °C, 0.1 M)(lit.)
useful pH range
7.0-9.0
solubility
water: soluble, clear
application(s)
diagnostic assay manufacturing
Application
Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Packaging
In convenient foil pouches
Preparation Note
Trizma solutions can be autoclaved.
Other Notes
Certain pH electrodes do not give accurate results with Tris buffers.
This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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G Travis Tabor et al.
Molecular and cellular neurosciences, 98, 121-130 (2019-06-19)
Kv4.2 voltage-gated K+ channel subunits, the primary source of the somatodendritic A-type K+ current in CA1 pyramidal neurons of the hippocampus, play important roles in regulating dendritic excitability and plasticity. To better study the trafficking and subcellular distribution of Kv4.2
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1194-10PAK | 04061837336850 |