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T1270

Thiamine hydrochloride

Name: Thiamine hydrochloride
Brand: SIGMA

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym(s):

Aneurine hydrochloride, Vitamin B₁ hydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₂H₁₇ClN₄OS · HCl

CAS Number:

67-03-8

Molecular Weight:

337.27

NACRES:

NA.75

PubChem Substance ID:

24899952

eCl@ss:

34058006

UNSPSC Code:

12352207

EC Number:

200-641-8

MDL number:

MFCD00012780

Beilstein/REAXYS Number:

3851771

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biological source

synthetic (organic)

Quality Level

200

product line

BioReagent

assay

≥99.0% (HPLC)

form

powder

mol wt

M_w 337.27 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

mp

250 °C (dec.) (lit.)

solubility

H₂O: 50 mg/mL, clear, colorless

application(s)

agriculture

SMILES string

CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]

InChI

1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1

InChI key

DPJRMOMPQZCRJU-UHFFFAOYSA-M

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Related Categories

Biochemicals

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

Molecular Biology & Functional Genomics

General description

Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain. Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:

of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii
of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis
of modified Bold 3N medium for culturing Chlorella minutissima

Biochem/physiol Actions

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase. Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway. Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome. Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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A convenient one-pot synthesis of formamide derivatives using thiamine hydrochloride as a novel catalyst

Lei M, et al.

Tetrahedron Letters, 51(32), 4186-4188 (2010)

PasT of Escherichia coli sustains antibiotic tolerance and aerobic respiration as a bacterial homolog of mitochondrial Coq10.

Cinzia Fino et al.

MicrobiologyOpen, 9(8), e1064-e1064 (2020-06-20)

Antibiotic-tolerant persisters are often implicated in treatment failure of chronic and relapsing bacterial infections, but the underlying molecular mechanisms have remained elusive. Controversies revolve around the relative contribution of specific genetic switches called toxin-antitoxin (TA) modules and global modulation of

Gene expression differences in infected and noninfected middle ear complementary DNA libraries.

Joseph E Kerschner et al.

Archives of otolaryngology--head & neck surgery, 135(1), 33-39 (2009-01-21)

To investigate genetic differences in middle ear mucosa (MEM) with nontypeable Haemophilus influenzae (NTHi) infection. Genetic upregulation and downregulation occurs in MEM during otitis media (OM) pathogenesis. A comprehensive assessment of these genetic differences using the techniques of complementary DNA

Improved growth and morphological plasticity of Haloferax volcanii.

Roshali T de Silva et al.

Microbiology (Reading, England), 167(2) (2021-01-19)

Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt to changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped ('plate') cells in culture, but may also be present as rods

Isolation and propagation of leptospires at 37 °C directly from the mammalian host.

Richard L Hornsby et al.

Scientific reports, 10(1), 9620-9620 (2020-06-17)

The causative agent of leptospirosis includes multiple serovars and species of pathogenic leptospires that are excreted via urine from reservoir hosts of infection. Primary isolation takes weeks to months, and is limited to semi-solid media at 28-30 °C. Here we present

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Thiamine in Cell Culture

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Global Trade Item Number

SKU	GTIN
T1270-25G	04061835569540
T1270-100G	04061835569533

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