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Merck
CN

T1283

holo-Transferrin bovine

Iron-saturated, BioReagent, suitable for cell culture

Synonym(s):

Siderophilin, Siderophilin, iron-saturated

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About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
234-318-8
NACRES:
NA.75
MDL number:
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Product Name

holo-Transferrin bovine, Iron-saturated, BioReagent, suitable for cell culture

product line

BioReagent

assay

97-100% (agarose gel electrophoresis)

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... TF(280705)

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Analysis Note

Purity by agarose gel electrophoresis.

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Bovine holo-transferrin can be used with a wide range of cells types. Bovine holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品
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Han Hu et al.
Animal science journal = Nihon chikusan Gakkaiho, 87(1), 84-91 (2015-10-16)
In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42°C for 0.5, 1, 3, 5, 8 or 12 h, and 38°C as control. The transcription of the genes (HSP27, HSP70 and
Samuel G Rodriques et al.
Nature biotechnology, 39(3), 320-325 (2020-10-21)
Current approaches to single-cell RNA sequencing (RNA-seq) provide only limited information about the dynamics of gene expression. Here we present RNA timestamps, a method for inferring the age of individual RNAs in RNA-seq data by exploiting RNA editing. To introduce
William Schachterle et al.
Nature communications, 8, 13963-13963 (2017-01-17)
Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little
Anastassia K Pogoutse et al.
Journal of bacteriology, 202(14) (2020-05-06)
The bacterial bipartite transferrin receptor is an iron acquisition system that several important human and animal pathogens require for survival. It consists of the TonB-dependent transporter transferrin binding protein A (TbpA) and the surface lipoprotein transferrin binding protein B (TbpB).
Wenting Dai et al.
The British journal of nutrition, 123(5), 489-498 (2019-11-13)
Despite the well-characterised mechanisms of amino acids (AA) regulation of milk protein synthesis in mammary glands (MG), the underlying specific AA regulatory machinery in bovine MG remains further elucidated. As methionine (Met) is one of the most important essential and

Articles

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。本页介绍转铁蛋白的体外化学和生物化学。

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