T1426
bovine pancreas
Proteomics Grade
essentially salt-free, lyophilized powder
≥10,000 BAEE units/mg protein
23.8 kDa
hydrochloric acid: soluble 1 mM
diagnostic assay manufacturing
Chymotrypsin ≤0.1 BTEE units/mg protein
−20°C
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Danger
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Respiratory system
11 - Combustible Solids
WGK 1
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
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Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).
Example:
Additional examples:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
enter as 1.000309185)
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How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Trypsin is soluble in 1 mM hydrochloric acid (pH approximately 3). It is also surprisingly stable in 1 mM HCl. Solutions are stable for approximately 1 year when aliquoted and stored at -20°C. The presence of Ca2+ (20 mM) will also retard trypsin's ability to autodigest, and will therefore help to maintain the stability of the trypsin in solution.
For trypsin digestion of peptides, use a ratio (w:w) of about 1:100 to 1:20 for trypsin:peptide. Trypsin preparations usually contain some contaminating chymotrypsin and this should be inhibited with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK). Product No. T1426 has already been treated with TPCK, so it does not need to be further treated.
Various values have been reported in the literature for the extinction coefficient of bovine pancreatic trypsin. The E1%(280) values have ranged from 12.9 - 15.4. When we assay the protein content of Product No. T1426 in our labs here at Sigma, we use a value of 14.4.
For in-gel digestions, we actually recommend using a methylated trypsin. Product No. T6567 is Trypsin from porcine pancreas, proteomics grade, dimethylated. For product T6567, the lysine residues of proteomics grade trypsin have been reductively methylated, resulting in a product that is resistant to autolysis. The T6567 has also been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized. This results in an enzyme that gives highly specific cleavage.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).
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