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Merck
CN

T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.51
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application(s)

CRISPR

shipped in

wet ice

storage temp.

−20°C

General description

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Features and Benefits

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Other Notes

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent
  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich® is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.
To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Regulatory Information

监管及禁止进口产品
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Aitziber Buqué et al.
Nature communications, 11(1), 3819-3819 (2020-08-01)
Hormone receptor (HR)+ breast cancer (BC) causes most BC-related deaths, calling for improved therapeutic approaches. Despite expectations, immune checkpoint blockers (ICBs) are poorly active in patients with HR+ BC, in part reflecting the lack of preclinical models that recapitulate disease

Protocols

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

可靠的PURedit™ CRISPR合成gRNA和Cas9蛋白可提供行业领先的靶位点切割和特异性

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默克Sigma-Aldrich® Advanced Genomics提供了一整套用于基因敲减和过表达实验的优化CRISPRi和CRISPRa文库目前可提供CRISPRi和SAM CRISPRa混合的慢病毒文库,也可根据您的特定需求提供定制产品。

The largest offering of quality Cas9 proteins ensures you have the perfect CRISPR reagents for your specific experimental needs. Rigorous purification, NLS signals, increased specificity and activity ensure your gene editing experiments are efficient and simple.

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

超丰富的优质 Cas9 蛋白组合,确保您始终可找到满足自身特定实验需求的完美 CRISPR 试剂。严苛纯化、NLS 信号、增强特异性和活性,确保您高效、简便地开展基因编辑实验。

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