Monoclonal Anti-Tumor Necrosis Factor Receptor II antibody produced in mouse

Anti-Tumor Necrosis Factor Receptor II antibody may be used for immunocytochemistry at a working concentration of 8-25 μg/ml using human peripheral blood lymphocytes. The antibody is suitable for neutralization assays and protein microarray. The antibody was used for flow cytometry using human CD8 T cells.

The antibody neutralizes the biological effect of recombinant human TNF RII. It does not neutralize or cross-react with TNF RI.

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The tumor necrosis factor super family (TNFSF) consists of at least 19 transmembrane proteins with soluble forms that are generated by proteolysis. The biological effects of TNF are mediated by either type I or type II receptors. Type I receptors that are ubiquitously present on all cells whereas type II receptors are expressed only on cells of immune system (monocytes, macrophages, Langerhans cells) and endothelial cells. Receptors of TNFSF such as TNFR I and Fas have their own death domains that activate the apoptosis. Other receptors such as TNFR II do not have death domains and utilize TNF associated factors (TRAFs) to activate intracellular signaling. TNFR II activates NF-κB pathway via the activation of NIK. Monoclonal Anti-Tumor Necrosis Factor Receptor II antibody recognizes human TNFR II.

recombinant human tumor necrosis factor receptor II, expressed in Escherichia coli.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Purified using protein A