T2400
Tropomyosin from porcine muscle
lyophilized powder (contains Tris buffer salts)
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About This Item
Form:
lyophilized powder (contains Tris buffer salts)
Assay:
≥70% protein basis (biuret)
Biological source:
Porcine muscle
biological source
Porcine muscle
Quality Segment
assay
≥70% protein basis (biuret)
form
lyophilized powder (contains Tris buffer salts)
technique(s)
electrophoresis: suitable
storage temp.
−20°C
General description
Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.
Application
Tropomyosin from porcine muscle has been used:
- as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
- in analyzing the anti-adhesive properties of fish tropomyosins
- in far Western blot analysis
Biochem/physiol Actions
Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.
Dimeric protein that assumes an α-helical coiled coil conformation. In the absence of Ca2+, inhibits muscle contractility by blocking the myosin binding sites on actin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.
Analysis Note
SDS electrophoresis shows two major bands.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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