Key Documents

View All Documentation

T2780

Monoclonal Anti-Tropomyosin antibody produced in mouse

Name: Monoclonal Anti-Tropomyosin antibody produced in mouse
Brand: SIGMA

clone TM311, ascites fluid

Synonym(s):

Anti-Tropomyosin Antibody

Select a Size

Change View

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00149029

Conjugate:

unconjugated

Clone:

TM311, monoclonal

Application:

ARR, ELISA (i), IF, IP, WB

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TM311, monoclonal

mol wt

antigen 36-39 kDa

contains

15 mM sodium azide

species reactivity

pig, chicken, bovine, rabbit, mouse, hamster, rat, human

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:400 using chicken fibroblasts, microarray: suitable, western blot: suitable using human tissue and chicken gizzard extracts

isotype

IgG1

UniProt accession no.

P09493

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... TPM1(281544)
chicken ... TPM1(396366)
human ... TPM1(7168)
mouse ... Tpm1(22003)
rat ... Tpm1(24851)

General description

Monoclonal Anti-Tropomyosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tropomyosin is a rigid rod-shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. Tropomyosin is made of two α helical polypeptide chains.

Immunogen

chicken gizzard tropomyosin.

Application

Monoclonal Anti-Tropomyosin has been used:

in immunofluorescent labelling
in immunohistochemistry
in western blotting
in immunoblotting

Mouse monoclonal clone TM311 anti-Tropomyosin antibody is used to tag tropomyosin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of tropomyosin in cytoskeletal structures.

Biochem/physiol Actions

Tropomyosin together with troponin, regulate the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help to confer structure and function to actins in the cytoskeleton and in striated muscle function.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动物来源生物产品

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tropomyosin-master regulator of actin filament function in the cytoskeleton

Gunning P W, et al.

Journal of Cell Science, 128(16), 2965-2974 (2015)

CacyBP/SIP as a novel modulator of the thin filament.

Ewelina Jurewicz et al.

Biochimica et biophysica acta, 1833(3), 761-766 (2012-12-26)

The CacyBP/SIP protein interacts with several targets, including actin. Since the majority of actin filaments are associated with tropomyosin, in this work we characterized binding of CacyBP/SIP to the actin-tropomyosin complex and examined the effects of CacyBP/SIP on actin filament

CENP-A is essential for cardiac progenitor cell proliferation

McGregor M, et al

Cell Cycle, 13(5), 739-748 (2014)

Vertebrate tropomyosin: distribution, properties and function

Perry, S Victor

Journal of Muscle Research and Cell Motility, 22(1), 5-49 (2001)

Non-canonical Wnt signaling enhances differentiation of Sca1+/c-kit+ adipose-derived murine stromal vascular cells into spontaneously beating cardiac myocytes

Palpant N J, et al.

Journal of Molecular and Cellular Cardiology, 43(3), 362-370 (2007)

View All Related Papers

Global Trade Item Number

SKU	GTIN
T2780-100UL	04061837726316
T2780-.2ML	04061837343209

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service