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Merck
CN

T2824

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-TNF-α

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD01632994

Key Documents

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Product Name

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster, ~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

biological source

hamster

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TN3-19.12, monoclonal

form

buffered aqueous solution

species reactivity

rat, mouse

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α

UniProt accession no.

P16599

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

mouse ... Tnf(21926)
rat ... Tnf(24835)

Related Categories

Application

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • neutralization

Biochem/physiol Actions

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.
TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.

Immunogen

purified recombinant mouse TNF-α.

Physical form

Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
118M4770V
118M4770V
038M4860V
064M4781
011M4788

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Cytokines, inflammation and pain
Zhang JM and An J
International Anesthesiology Clinics, 45(2), 27-27 (2007)
Role of the Adipocyte in Metabolism and Endocrine Function
Endocrinology: Adult and Pediatric (2016)

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