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T2885

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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100 MG
CN¥390.22
1 G
CN¥464.71
5 G
CN¥1,810.03
100 G
CN¥29,143.73

About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
Beilstein:
2808541
EC Number:
259-364-6
MDL number:
MFCD00007748
UNSPSC Code:
12352204
PubChem Substance ID:
24900090
NACRES:
NA.83

CN¥390.22

Available to ship onOctober 14, 2025Details

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Product Name

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

Assay

≥98% (TLC)

form

powder

mp

168-171 °C (lit.)

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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This Item
86033687748T5525
3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)

Sigma-Aldrich

T2885

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥99%

Sigma-Aldrich

860336

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)

Sigma-Aldrich

87748

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet

Sigma-Aldrich

T5525

3,3′,5,5′-Tetramethylbenzidine

assay

≥98% (TLC)

assay

≥99%

assay

≥98.0% (NT)

assay

-

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

powder

form

powder

form

powder

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

168-171 °C (lit.)

mp

168-171 °C (lit.)

mp

166-171 °C, 168-171 °C (lit.)

mp

168-171 °C (lit.)

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used:
  • as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2[1][2]
  • as a substrate solution for ELISA (enzyme-linked immunosorbent assay)[3]
  • in myeloperoxidase activity assay to quantify neutrophil[4]

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction.[5] In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.[6]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302,H351,H413

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    WXBF5971V
    WXBF5441V
    WXBF2547V
    WXBF4932V
    WXBF3620V

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    Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system
    Methods in Neurosciences, 21(1), 93-117 (1994)
    Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
    Yamaoka H, et al.
    Letters in Applied Microbiology, 62(1), 47-54 (2016)
    Lactobacillus casei improves resistance to pneumococcal respiratory infection in malnourished mice
    Villena J, et al.
    The Journal of Nutrition, 135(6), 1462-1469 (2005)
    The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk
    Sayi A, et al.
    Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)
    Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle
    Rodriguez C, et al.
    Anaerobe, 17(1), 15-18 (2011)
    View All Related Papers

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