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Merck
CN

T3160

TC-100 Insect Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Synonym(s):

BML-TC/10

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Technical Service
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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

glucose: 1.0 g/L
L-glutamine: 0.6 g/L
NaHCO3: 0.35 g/L

shipped in

ambient

storage temp.

2-8°C

General description

TC-100 Insect Medium has been developed to optimize the production of Autographica californica nuclear polyhedrosis virus (NPV) virions by cells from the fall armyworm, Spodoptera frugiperda. It has been found to support the growth of cells derived from a variety of lepidopteran species. The medium is mostly employed in the growth of baculoviruses in lepidopteran cell lines.

Application

TC-100 Insect Medium has been used:
  • in the transfection of insect cells in suspension for efficient baculovirus generation
  • to harvest Varroa destructor saliva
  • as a starter media to culture and maintain insect cells such as Spodoptera frugiperda

For baculovirus culturing in lepidoptera.


Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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