T4019
Trypsin−Agarose
buffered aqueous suspension, from bovine pancreas trypsin
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About This Item
Biological source:
bovine pancreas (trypsin)
Concentration:
≥15 units/mL (packed gel)
biological source
bovine pancreas (trypsin)
Quality Segment
form
buffered aqueous suspension
concentration
≥15 units/mL (packed gel)
extent of labeling
≥15 units per mL (packed gel)
matrix
beaded agarose
storage temp.
2-8°C
General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Physical form
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Preparation Note
TPCK-treated
Other Notes
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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