Skip to Content
Merck
CN

T4510

Thymidine 5′-monophosphate p-nitrophenyl ester sodium salt

chromogenic, ≥98% (HPLC), powder

Synonym(s):

p-Nitrophenyl-5V-thymidine-monophosphate, p-Nph-5V-TMP

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C16H18N3O10PNa
CAS Number:
98179-10-3
Molecular Weight:
466.29
UNSPSC Code:
12352204
PubChem Substance ID:
131888503
NACRES:
NA.32

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Thymidine 5′-monophosphate p-nitrophenyl ester sodium salt, ≥98% (HPLC)

SMILES string

CC(C(N1)=O)=CN([C@H]2C[C@H](O)[C@@H](COP(O)(OC(C=C3)=CC=C3[N+]([O-])=O)=O)O2)C1=O.[Na]

InChI

1S/C16H18N3O10P.Na.H/c1-9-7-18(16(22)17-15(9)21)14-6-12(20)13(28-14)8-27-30(25,26)29-11-4-2-10(3-5-11)19(23)24;;/h2-5,7,12-14,20H,6,8H2,1H3,(H,25,26)(H,17,21,22);;

InChI key

PHNRUCBIIRECII-UHFFFAOYSA-N

assay

≥98% (HPLC)

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

Quality Level

200

Related Categories

General description

A sensitive chromogenic substrate for venom phosphodiesterase. It is not hydrolyzed by bovine spleen phosphodiesterase.

Application

Thymidine 5′-monophosphate p-nitrophenyl ester sodium salt has been used as a substrate for assessing phosphodiesterase activity. It is also been used as a nucleotide analog in the autotaxin inhibition assay.

Biochem/physiol Actions

Thymidine 5′-monophosphate p-nitrophenyl ester sodium salt is an artificial substrate marker for 5′-nucleotide phosphodiesterase enzyme activity. It is also used as a substrate for autotaxin (ATX, NPP2), which is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (NPP) enzyme family.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000527785
0000527785
0000523660
0000523660
0000520178

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Adenosine A2A receptor agonist (CGS-21680) prevents endotoxin-induced effects on nucleotidase activities in mouse lymphocytes.
Vuaden FC, et al.
European Journal of Pharmacology, 651(1-3), 212-217 (2011)
Characterization of non-lipid autotaxin inhibitors.
Hoeglund AB, et al.
Bioorganic & Medicinal Chemistry, 18(2), 769-776 (2010)
Paul R Stabach et al.
Clinical and translational science, 14(1), 362-372 (2020-10-17)
Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy. We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a
Soluble NTPDase: an additional system of nucleotide hydrolysis in rat blood serum.
Oses JP, et al.
Life Sciences, 74(26), 3275-3284 (2004)
Annie C Chou et al.
The Journal of biological chemistry, 294(8), 2665-2677 (2018-12-30)
Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a multifunctional protein that has been implicated in a myriad of cellular pathways. Although most well-known for its phosphodiesterase activity removing stalled topoisomerase 2 from DNA, TDP2 has also been shown to interact with both
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service