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T5574

Terrific Broth

Name: Terrific Broth
Brand: SIGMA

Liquid microbial growth medium

Synonym(s):

TB medium, Tartoff – Hobbs medium

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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Quality Level

500

grade

Molecular Biology

description

Liquid Medium

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

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Related Categories

Cloning & Expression

Molecular Cloning & Protein Expression

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Terrific Broth liquid provides:

Filter-sterilized, ready-to-use format
Convenient package size
Standard formulation

Other Notes

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K₂HPO₄
2.2g/L KH₂PO₄
4ml/L Glycerol

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

动物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number

0000407886

0000408192

SLCN1739

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Combinatorial method for overexpression of membrane proteins in Escherichia coli.

Shani Leviatan et al.

The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)

Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for

The preparation and analysis of DNA for use in transgenic technology.

Auerbach, A.B., et al.

Advanced Protocols For Animal Transgenesis, 501-501 (2011)

High yield expression and purification of HIV-1 Tat1-72 for structural studies.

Shaheen Shojania et al.

Journal of virological methods, 164(1-2), 35-42 (2009-11-28)

The HIV-1 transactivator of transcription (Tat) is a protein essential for virus replication. Tat is an intrinsically disordered RNA-binding protein that, in cooperation with host cell factors cyclin T1 and cyclin-dependent kinase 9, regulates transcription at the level of elongation.

Swimming bacteria power microscopic gears.

Andrey Sokolov et al.

Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)

Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class

Kinetic characterization of VIM-7, a divergent member of the VIM metallo-beta-lactamase family.

Ørjan Samuelsen et al.

Antimicrobial agents and chemotherapy, 52(8), 2905-2908 (2008-06-19)

Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible

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Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Global Trade Item Number

SKU	GTIN
T5574-500ML	04061837347993
CR40057-1000M	04061838445797

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