T7039
Transforming Growth Factor-β1 human
TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture
Synonym(s):
hTGF-β1, TGF-β1
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About This Item
Biological source:
human
Recombinant:
expressed in CHO cells
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
dimer (12.8/25.6 kDa (112/224 aa))
Impurities:
≤0.1 EU/μg (LAL test)
biological source
human
Quality Level
recombinant
expressed in CHO cells
assay
≥95% (SDS-PAGE)
form
powder
potency
≤0.5 ng/mL ED50
mol wt
dimer (12.8/25.6 kDa (112/224 aa))
packaging
pkg of 2 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤0.1 EU/μg (LAL test)
UniProt accession no.
storage temp.
−20°C
General description
Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.
Application
Transforming Growth Factor-β1 human has been used in
- reporter gene assay.
- in vitro transfection.
- to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
- to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
- to study the interaction between TGF-β1 activation and myofibroblast formation.
Biochem/physiol Actions
TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
Physical form
Lyophilized from a 0.2 µm filtered solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio
Preparation Note
Reconstitution: Sterile 10 mM HCl at 0.1 mg/mL
Analysis Note
The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
监管及禁止进口产品
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Articles
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| T7039-50UG | 04061837372254 |
| T7039-2UG | 04061835569663 |